Delft University of Technology, Kavli Institute of Nanoscience, Department of Bionanoscience, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Curr Opin Cell Biol. 2012 Feb;24(1):100-6. doi: 10.1016/j.ceb.2011.11.011. Epub 2011 Dec 22.
The nuclear pore complex (NPC) has long been viewed as a point-like entry and exit channel between the nucleus and the cytoplasm. New data support a different view whereby the complex displays distinct spatial dynamics of variable duration ranging from milliseconds to events spanning the entire cell cycle. Discrete interaction sites outside the central channel become apparent, and transport regulation at these sites seems to be of greater importance than currently thought. Nuclear pore components are highly active outside the NPC or impact the fate of cargo transport away from the nuclear pore. The NPC is a highly dynamic, crowded environment-constantly loaded with cargo while providing selectivity based on unfolded proteins. Taken together, this comprises a new paradigm in how we view import/export dynamics and emphasizes the multiscale nature of NPC-mediated cellular transport.
核孔复合体（NPC）长期以来一直被视为细胞核和细胞质之间的点状进出口通道。新的数据支持了一种不同的观点，即该复合物显示出不同的空间动力学，持续时间从毫秒到跨越整个细胞周期的事件不等。中央通道外的离散相互作用位点变得明显，而这些位点的运输调节似乎比目前认为的更为重要。核孔组件在 NPC 之外非常活跃，或者影响货物运输离开核孔的命运。NPC 是一个高度动态、拥挤的环境——不断装载货物，同时根据未折叠的蛋白质提供选择性。总的来说，这构成了我们看待进出口动力学的新范式，并强调了 NPC 介导的细胞运输的多尺度性质。
