Goebel S J, Johnson G P, Perkus M E, Davis S W, Winslow J P, Paoletti E
Virogenetics Corporation, Troy, New York 12180-8349.
Virology. 1990 Nov;179(1):247-66, 517-63. doi: 10.1016/0042-6822(90)90294-2.
The complete DNA sequence of the genome of vaccinia virus has been determined. The genome consisted of 191,636 bp with a base composition of 66.6% A + T. We have identified 198 "major" protein-coding regions and 65 overlapping "minor" regions, for a total of 263 potential genes. Genes encoded by the virus were located by examination of DNA sequence characteristics and compared with existing vaccinia virus mapping analyses, sequence data, and transcription data. These genes were found to be compactly organized along the genome with relatively few regions of noncoding sequences. Whereas several similarities to proteins of known function were discerned, the function of the majority of proteins encoded by these open reading frames is as yet undetermined.
痘苗病毒基因组的完整DNA序列已被确定。该基因组由191,636个碱基对组成，碱基组成中A + T占66.6%。我们已鉴定出198个“主要”蛋白质编码区和65个重叠的“次要”区域，总共263个潜在基因。通过检查DNA序列特征来定位病毒编码的基因，并与现有的痘苗病毒图谱分析、序列数据和转录数据进行比较。发现这些基因沿着基因组紧密排列，非编码序列区域相对较少。虽然识别出了与已知功能蛋白质的一些相似性，但这些开放阅读框编码的大多数蛋白质的功能尚未确定。
