Schmitt J F, Stunnenberg H G
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
J Virol. 1988 Jun;62(6):1889-97. doi: 10.1128/JVI.62.6.1889-1897.1988.
The complete sequence of the vaccinia virus HindIII I fragment, which is composed of 6,498 base pairs, encodes six complete and two incomplete open reading frames (ORFs). Computer analysis revealed an amino acid sequence homology between ORF I 4 and the large subunit of the ribonucleotide reductase complex. The two small polypeptides derived from ORFs I 2 and I 5, with molecular weights of 8,500 and 8,700, respectively, have a very high hydrophobic amino acid sequence composition. S1 analysis revealed that ORF I 4 is expressed at early stages of infection, ORFs I 1, I 2, I 5, and I 7 are expressed in the late phase of infection, and ORF I 3 is constitutively expressed. Screening a vaccinia virus genomic library revealed a large vaccinia virus insert overlapping the HindIII I and O fragments which contains a previously undetected HindIII P fragment of approximately 300 base pairs. S1 analysis revealed an early (O1) and a late (O2) start site of transcription initiation located within the HindIII O fragment.
痘苗病毒HindIII I片段由6498个碱基对组成,其完整序列编码六个完整的和两个不完整的开放阅读框(ORF)。计算机分析显示,ORF I 4与核糖核苷酸还原酶复合体的大亚基之间存在氨基酸序列同源性。由ORF I 2和I 5衍生的两个小多肽,分子量分别为8500和8700,具有非常高的疏水性氨基酸序列组成。S1分析表明,ORF I 4在感染早期表达,ORF I 1、I 2、I 5和I 7在感染后期表达,ORF I 3组成性表达。筛选痘苗病毒基因组文库发现一个大的痘苗病毒插入片段,它与HindIII I和O片段重叠,其中包含一个约300个碱基对的先前未检测到的HindIII P片段。S1分析显示,转录起始的早期(O1)和晚期(O2)起始位点位于HindIII O片段内。