Department of General Biochemistry, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Clin Biochem. 2012 Feb;45(3):231-6. doi: 10.1016/j.clinbiochem.2011.12.002. Epub 2011 Dec 19.
In breast cancer patients dysregulation of reactive oxygen species metabolism, as detected by various indicators in plasma or various blood cells, including red blood cells and blood platelets, is observed.
The aim of our study was to explain the effect of oxidative/nitrative stress on hemostatic activity of plasma from breast cancer patients. Different methods were used to analyze oxidative/nitrative stress (carbonyl groups and 3-nitrotyrosine levels - ELISA and C-ELISA method, respectively and free thiol groups was estimated with 5,5'-dithio-bis(2-nitro-benzoic acid)). We also measured total antioxidant status (TAS) in plasma.
Our results showed the correlation between the oxidative stress and changes of hemostasis in breast cancer patients; the correlation between the increased amount of carbonylated or nitrated plasma proteins and fibrin polymerization or lysis in plasma isolated from invasive breast cancer patients. We also observed that the relationship between TAS and fibrin polymerization or lysis in plasma from invasive breast cancer exists.
Considering the data presented in this study, we suggest that the oxidative/nitrative stress in breast cancer patients may induce the changes of hemostasis in these patients.
在乳腺癌患者中，观察到活性氧代谢失调，这可通过血浆或各种血细胞（包括红细胞和血小板）中的各种指标检测到。
本研究旨在解释氧化/硝化应激对乳腺癌患者血浆止血活性的影响。我们使用不同的方法来分析氧化/硝化应激（羰基和 3-硝基酪氨酸水平——ELISA 和 C-ELISA 方法，分别用 5,5'-二硫代双（2-硝基苯甲酸）估计游离巯基）。我们还测量了血浆中的总抗氧化状态（TAS）。
我们的结果表明，氧化应激与乳腺癌患者止血变化之间存在相关性；在从浸润性乳腺癌患者中分离的血浆中，血浆中羰基化或硝化蛋白的数量增加与纤维蛋白聚合或纤溶之间存在相关性。我们还观察到，TAS 与来自浸润性乳腺癌患者的血浆中纤维蛋白聚合或纤溶之间存在关系。
考虑到本研究中提供的数据，我们认为乳腺癌患者中的氧化/硝化应激可能会引起这些患者的止血变化。
