Kikuchi Haruhisa, Miyagawa Yasuhiro, Sahashi Yuko, Inatomi Satoshi, Haganuma Asami, Nakahata Norimichi, Oshima Yoshiteru
Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-yama, Aoba-ku, Sendai 980-8578, Japan.
J Org Chem. 2004 Jan 23;69(2):352-6. doi: 10.1021/jo035137x.
Entomopathogenic fungi forming fruiting bodies have been employed as tonics and antitussives from ancient times. Paecilomyces tenuipes, which is also called Isaria japonica, is a very popular entomopathogenic fungus and is often considered a health food in northeast Asian countries such as China, Korea, and Japan. We cultivated the fruiting bodies of Paecilomyces tenuipes. Among the large-scale cultivations, fruiting body grown in barley grain contained two novel spirocyclic trichothecane derivatives, spirotenuipesine A (1) and B (2), and known trichothecane mycotoxins. Compounds 1 and 2 showed potent activity in neurotrophic factor biosynthesis in glial cells. The isolation of these compounds indicated that P. tenuipes is a promising source for producing various biologically active substances including trichothecanes. It is noteworthy that trichothecane mycotoxins are present in Paecilomyces tenuipes, which is typically used in medicinal health food.
自古以来，形成子实体的昆虫病原真菌就被用作滋补品和止咳药。细脚拟青霉，也被称为日本棒束孢，是一种非常受欢迎的昆虫病原真菌，在中国、韩国和日本等东北亚国家常被视为健康食品。我们培养了细脚拟青霉的子实体。在大规模培养中，在大麦粒上生长的子实体含有两种新型螺环单端孢霉烯衍生物，细脚拟青霉螺环素A（1）和B（2），以及已知的单端孢霉烯霉菌毒素。化合物1和2在神经胶质细胞的神经营养因子生物合成中显示出强效活性。这些化合物的分离表明，细脚拟青霉是生产包括单端孢霉烯类在内的各种生物活性物质的有前途的来源。值得注意的是，单端孢霉烯霉菌毒素存在于通常用于药用保健食品的细脚拟青霉中。
