Azadi Mostafa D A, Hassanajili Shadi, Zarrabi Khalil, Sarkari Bahador
Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.
Department of Cardiovascular Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Prog Biomater. 2018 Mar;7(1):35-54. doi: 10.1007/s40204-018-0082-5. Epub 2018 Feb 19.
Cystic echinococcosis (CE)/hydatid cyst is one of the most important helminthic diseases in the world. The treatment of hydatid cyst ranges from surgical intervention to chemotherapy, although the efficacy of chemotherapy is still unclear. Postoperative complication which results from the spillage of cysts during surgical operation is one of the most important concerns in surgical treatment of hydatid cyst. The aim of the current study was to solidify the hydatid cyst fluid (HCF) with an injectable and thermosensitive chitosan (CS)/carboxymethyl cellulose (CMC)/β-glycerol phosphate (BGP) hydrogel for effective control of spillage during the aspiration of hydatid cysts. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water uptake, rheological analysis, and Alamar Blue cytotoxicity assay were employed to characterize the hydrogel. A five level with three times replication at the central point using a central composite design (CCD), which is a response surface methodology (RSM), was used to optimize the experimental conditions. Assessment of the produced hydrogel showed that the intermolecular interactions of amino groups of chitosan and hydrogen groups of CMC were correctively established and appreciable swelling with a good strength was obtained. Hydrogels morphology had a porous structure. Rheological analysis showed that CS/CMC/BGP blends had a phase transition (32-35 °C) of sol-gel close to the body temperature. Alamar Blue cytotoxicity assay showed that CS (1.75%)/CMC (1.4%)/BGP (2.9%) had IC50 values of 0.598, 0.235 and 0.138 (µg/µL) for 24, 48 and 72 h, which indicated that the produced polymer solution had no significant cytotoxic effect for human fibroblast cell line. In vitro injection of the polymer solution of CS/CMC/BGP with CS/CMC ratio of 1.75/1.4 was done on HCF (1 mL polymer solution to 3 mL of HCF) at 37 °C with a final concentration of 2.9% for BGP resulting in solidification of HCF in less than 45 min.
囊型包虫病（CE）/包虫囊肿是世界上最重要的蠕虫病之一。包虫囊肿的治疗方法包括手术干预和化疗，不过化疗的疗效仍不明确。手术过程中囊肿破裂导致的术后并发症是包虫囊肿手术治疗中最重要的问题之一。本研究的目的是用一种可注射的热敏壳聚糖（CS）/羧甲基纤维素（CMC）/β-甘油磷酸酯（BGP）水凝胶固化包虫囊肿液（HCF），以有效控制包虫囊肿抽吸过程中的渗漏。采用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、吸水率、流变学分析和阿拉玛蓝细胞毒性试验对水凝胶进行表征。使用中心复合设计（CCD）（一种响应面方法（RSM））在中心点进行五水平三次重复实验，以优化实验条件。对制备的水凝胶的评估表明，壳聚糖的氨基与CMC的氢基团之间的分子间相互作用得以正确建立，并且获得了具有良好强度的可观溶胀。水凝胶形态具有多孔结构。流变学分析表明，CS/CMC/BGP共混物的溶胶-凝胶相转变温度（32 - 35°C）接近体温。阿拉玛蓝细胞毒性试验表明，CS（1.75%）/CMC（1.4%）/BGP（2.9%）在24、48和72小时的IC50值分别为0.598、0.
