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用于皮肤评估的多光子激光显微镜和荧光寿命成像

Multiphoton laser microscopy and fluorescence lifetime imaging for the evaluation of the skin.

作者信息

Seidenari Stefania, Arginelli Federica, Bassoli Sara, Cautela Jennifer, French Paul M W, Guanti Mario, Guardoli Davide, König Karsten, Talbot Clifford, Dunsby Chris

机构信息

Department of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, Italy.

出版信息

Dermatol Res Pract. 2012;2012:810749. doi: 10.1155/2012/810749. Epub 2011 Nov 28.

DOI:10.1155/2012/810749
PMID:22203841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3235701/
Abstract

Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity emitted by epidermal and dermal fluorophores and by the extra-cellular matrix, fluorescence lifetime imaging (FLIM), is generated by the fluorescence decay rate. This innovative technique can be applied to the study of living skin, cell cultures and ex vivo samples. Although still limited to the clinical research field, the development of multiphoton laser microscopy is thought to become suitable for a practical application in the next few years: in this paper, we performed an accurate review of the studies published so far, considering the possible fields of application of this imaging method and providing high quality images acquired in the Department of Dermatology of the University of Modena.

摘要

多光子激光显微镜是一种新型的非侵入性技术，可在细胞和亚细胞水平上观察皮肤，它基于自发荧光和荧光寿命成像。前者考虑表皮和真皮荧光团以及细胞外基质发出的荧光强度，而荧光寿命成像（FLIM）则由荧光衰减率产生。这种创新技术可应用于活体皮肤、细胞培养和离体样本的研究。尽管多光子激光显微镜仍局限于临床研究领域，但人们认为在未来几年它将适用于实际应用：在本文中，我们对迄今为止发表的研究进行了精确回顾，考虑了这种成像方法可能的应用领域，并提供了在摩德纳大学皮肤科获取的高质量图像。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/aefd364c5230/DRP2012-810749.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/fe7055c17589/DRP2012-810749.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/99b534ceaa3d/DRP2012-810749.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/0b59b884f02f/DRP2012-810749.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/ca651708045a/DRP2012-810749.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/0697b2a975bd/DRP2012-810749.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/d54d80a55c81/DRP2012-810749.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/b153b513195b/DRP2012-810749.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/69941c744245/DRP2012-810749.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/aefd364c5230/DRP2012-810749.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/fe7055c17589/DRP2012-810749.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/99b534ceaa3d/DRP2012-810749.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/0b59b884f02f/DRP2012-810749.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/ca651708045a/DRP2012-810749.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/0697b2a975bd/DRP2012-810749.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/d54d80a55c81/DRP2012-810749.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/b153b513195b/DRP2012-810749.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/69941c744245/DRP2012-810749.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11ca/3235701/aefd364c5230/DRP2012-810749.009.jpg

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