Grupo de Estudos em Pneumocystis, Serviço de Infectologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Med Mycol. 2012 Jul;50(5):556-60. doi: 10.3109/13693786.2011.645892. Epub 2011 Dec 29.
A high rate of Pneumocystis jirovecii colonization was observed in Brazilian cystic fibrosis (CF) patients (13 out of 34; 38.2%) who underwent bronchoscopy between March 2006 and August 2009 at the Hospital de Clinicas de Porto Alegre, Brazil. Bronchoalveolar lavage samples were collected from these patients and studied by nested PCR amplification of the mitochondrial gene coding for the large subunit ribosomal RNA (mtLSUrDNA). The observed rate of colonization was higher than that reported in European populations. Genotypic characterization of the mtLSUrDNA locus revealed a predominance of the polymorphisms 85C/248C (genotype 1) and 85T/248C (genotype 3), with all samples possessing the wild-type genotype of dihydropteroate synthase. These findings suggest that cystic fibrosis patients could be an important reservoir and source of P. jirovecii infection. Further studies are required to elucidate the role of this common fungal colonization in the evolution of CF patients.
在巴西阿雷格里港临床医院，2006 年 3 月至 2009 年 8 月间，对 34 名囊性纤维化（CF）患者进行支气管镜检查，其中 13 名（38.2%）患者存在耶氏肺孢子菌定植。从这些患者的支气管肺泡灌洗液样本中提取 DNA，采用巢式 PCR 扩增线粒体编码大亚基核糖体 RNA（mtLSUrDNA）基因，对其进行研究。观察到的定植率高于欧洲人群的报道。mtLSUrDNA 基因座的基因型特征显示，85C/248C（基因型 1）和 85T/248C（基因型 3）的多态性占主导地位，所有样本均具有二氢叶酸合成酶的野生型基因型。这些发现表明，囊性纤维化患者可能是耶氏肺孢子菌感染的重要储主和传染源。需要进一步研究以阐明这种常见真菌定植在 CF 患者中的作用。
