P63和Ki-67在根端囊肿中的表达

P63 and Ki-67 expression in radicular cyst.

作者信息

Alsaegh Mohammed Amjed, Mahmoud Okba, Varma Sudhir Rama, Mathew Asok, Altaie Alaa Muayad, Zhu Shengrong

机构信息

Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates.

Department of Clinical Sciences, College of Dentistry, Ajman University, Ajman, United Arab Emirates.

出版信息

J Oral Biol Craniofac Res. 2023 Sep-Oct;13(5):575-580. doi: 10.1016/j.jobcr.2023.06.008. Epub 2023 Jul 29.

DOI:10.1016/j.jobcr.2023.06.008

PMID:37545663

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10403738/

Abstract

OBJECTIVES

The aim of the current study was to identify the expression of P63 and its relation to odontogenic epithelial cell proliferation, severity of the inflammatory infiltrate and size of radicular cysts (RCs).

METHODS

In this retrospective cross-sectional study, 30 cases of paraffin-embedded RCs were randomly selected from the archive. P63 and Ki-67 expression was assessed by immunohistochemistry.

RESULTS

Epithelial P63 expression was absent in four (13.3%), weak in 10 (33.3%), and moderate in 16 (53.3%) cases. In the connective tissue wall of RC, P63 expression was absent in two (6.7%) cases, weak in 24 (80.0%) cases, and moderate in four (13.3%) cases. Ki-67 was found to be weakly expressed in 12 (40.0%) cases, moderately expressed in 13 (43.3%), and strongly expressed in five (16.7%) cases. No correlation was found between Ki-67 expression in odontogenic epithelium and P63 expression in the odontogenic epithelium (rho = 0.110, p = .563) or fibrous capsule (rho = 0.160, p = .399). Nevertheless, we found a positive correlation between Ki-67 expression in the odontogenic epithelium and the size of the RC (rho = 0.450, p = .013). The inflammatory infiltrate was negatively correlated with P63 expression in the odontogenic epithelium (rho = -0.428, p = .018), and with the size of cysts (rho = -0.728, p < .001).

CONCLUSIONS

There is a high expression of P63 throughout the odontogenic epithelium and connective tissue capsule of the RC. P63 expression in the odontogenic epithelium is negatively correlated with the degree of the inflammatory infiltrate but not with epithelial cell proliferation or the size of the cyst.

摘要

目的

本研究旨在确定P63的表达及其与牙源性上皮细胞增殖、炎性浸润严重程度和根囊肿（RCs）大小的关系。

方法

在这项回顾性横断面研究中，从存档中随机选取30例石蜡包埋的RCs。通过免疫组织化学评估P63和Ki-67的表达。

结果

上皮P63表达在4例（13.3%）中缺失，10例（33.3%）中为弱阳性，16例（53.3%）中为中等强度。在RC的结缔组织壁中，P63表达在2例（6.7%）中缺失，24例（80.0%）中为弱阳性，4例（13.3%）中为中等强度。发现Ki-67在12例（40.0%）中弱表达，13例（43.3%）中中等表达，5例（16.7%）中强表达。在牙源性上皮中Ki-67表达与牙源性上皮中P63表达（rho = 0.110，p = 0.563）或纤维囊（rho = 0.160，p = 0.399）之间未发现相关性。然而，我们发现牙源性上皮中Ki-67表达与RC大小之间存在正相关（rho = 0.450，p = 0.013）。炎性浸润与牙源性上皮中P63表达呈负相关（rho = -0.428，p = 0.018），与囊肿大小呈负相关（rho = -0.728，p < 0.001）。