School of Food Science and Technology, Chung-Ang University, 72-1 Nae-Ri, Daeduck-Myun, Ansung, Gyunggido 456-756, Korea.
J Food Prot. 2012 Jan;75(1):184-8. doi: 10.4315/0362-028X.JFP-11-243.
This study was conducted to determine the concentration and optimal treatment time of chlorine for reducing feline calicivirus (FCV) and murine norovirus (MNV) as surrogates of norovirus (NoV) on stainless steel surfaces and to develop a predictive inactivation method using a response surface methodology. The reduction levels of FCV VR-782 and MNV on stainless steel surfaces after treatment with various concentrations of chlorine (0 to 5,000 ppm) for various times (0 to 5 min) were measured. The reduction values of both FCV and MNV on stainless steel surfaces after 5,000 ppm of chlorine treatment for 5 min were 5.20 TCID(50) per coupon. The predictive results obtained by central composite design were analyzed by standard analysis of variance. The application of multiple regression analysis was related to the following polynomial equations: (i) FCV (log TCID(50) per coupon) = -0.3714 + 0.8362x(1) + 0.0011x(2) + 0.0001x(1)x(2) - 0.1143x(2)(1) -0.0001x(2)(2) (x(1), time; x(2), concentration) and (ii) MNV (log TCID(50) per coupon) = + 0.0471 + 0.0807x(1) + 0.0011x(2) + 0.0001x(1)x(2) -0.0910x(2)(1) -0.0001x(2)(2) (x(1), time; x(2), concentration). It was concluded that these polynomial equation models of reduction of FCV and MNV could be used to determine the minimum concentration of chlorine and exposure times to control human NoV on food contact surfaces.
本研究旨在确定氯的浓度和最佳处理时间，以降低不锈钢表面猫杯状病毒（FCV）和鼠诺如病毒（MNV）作为诺如病毒（NoV）的替代物，并利用响应面法开发预测失活动力学方法。测量了不同浓度（0 至 5000ppm）氯处理不同时间（0 至 5 分钟）后，FCV VR-782 和 MNV 在不锈钢表面的减少水平。5000ppm 氯处理 5 分钟后，FCV 和 MNV 在不锈钢表面的减少值均为每个小格 5.20TCID(50)。通过中心复合设计获得的预测结果通过标准方差分析进行分析。多元回归分析的应用与以下多项式方程相关：（i）FCV（每个小格的对数 TCID(50)）=-0.3714+0.8362x(1)+0.0011x(2)+0.0001x(1)x(2)-0.1143x(2)(1)-0.0001x(2)(2)（x(1)，时间；x(2)，浓度）和（ii）MNV（每个小格的对数 TCID(50)）=+0.0471+0.0807x(1)+0.0011x(2)+0.0001x(1)x(2)-0.0910x(2)(1)-0.0001x(2)(2)（x(1)，时间；x(2)，浓度）。结论是，这些 FCV 和 MNV 减少的多项式方程模型可用于确定控制食品接触面人诺如病毒的最低氯浓度和暴露时间。
