Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Molecules. 2012 Jan 5;17(1):504-10. doi: 10.3390/molecules17010504.
Our ongoing investigations on the stem bark of Uncaria macrophylla afforded a new ursolic triterpene, 3β,6β,19α-trihydroxy-urs-12-en-28-oic acid-24-carboxylic acid methyl ester (1), named uncariursanic acid, and three known ursolic triterpenes including 3β,6β,19α-trihydroxy-23-oxo-urs-12-en-28-oic acid (2), 3β,6β,19α-trihydroxy-urs-12-en-28-oic acid (3) and ursolic acid (4). Their structures were elucidated by extensive spectral methods, including 1D and 2D NMR and HR-ESI-MS. The cytotoxicities of the four compounds were evaluated against two cancer cell lines (MCF-7 and HepG2) by the MTT method, and only compound 4 exhibited potent activity.
我们对钩藤茎皮的持续研究得到了一种新的熊果酸三萜，3β,6β,19α-三羟基-熊-12-烯-28-酸-24-羧酸甲酯（1），命名为钩藤熊果酸，以及三种已知的熊果酸三萜，包括 3β,6β,19α-三羟基-23-氧代-熊-12-烯-28-酸（2）、3β,6β,19α-三羟基-熊-12-烯-28-酸（3）和熊果酸（4）。它们的结构通过广泛的光谱方法，包括 1D 和 2D NMR 和 HR-ESI-MS 来阐明。四种化合物的细胞毒性通过 MTT 法在两种癌细胞系（MCF-7 和 HepG2）中进行评估，只有化合物 4 表现出很强的活性。
