Ashworth L A, Lloyd G, Baskerville A
Arch Virol. 1979;59(4):307-18. doi: 10.1007/BF01317471.
Antibody-dependent cell-mediated cytotoxicity (ADCC) was studied using as targets 51Cr-labelled Vero cells infected with the Bartha strain of Aujeszky's disease virus (ADV). Using hyperimmune anti-ADV serum to sensitize the targets, porcine leukocytes from dextran-sedimented blood were found to be efficient effector cells yielding maximal 51Cr release by 16 hours. Whilst complement-dependent cytotoxic antibody could be demonstrated no enhancement of ADCC by complement was found. The sera of pigs vaccinated i.m. with Bartha virus were titrated in ADCC using leukocytes as effector cells and the results compared with those obtained by virus neutralization. ADCC proved to be a much more sensitive technique and might, therefore, provide the basis for a reliable diagnostic test. Partially purified lymphocytes and polymorphonuclear leukocytes from blood and peritoneal exudates, and macrophages from exudates were found to mediate ADCC with hyperimmune serum, but differences were observed in the efficiency and timing of their cytotoxic effects.
利用51Cr标记的、感染了奥耶斯基氏病病毒（ADV）巴塔株的Vero细胞作为靶标，研究了抗体依赖的细胞介导的细胞毒性（ADCC）。使用超免疫抗ADV血清使靶标致敏，发现来自葡聚糖沉淀血液的猪白细胞是有效的效应细胞，在16小时时可产生最大的51Cr释放。虽然可以证明补体依赖的细胞毒性抗体，但未发现补体对ADCC有增强作用。使用白细胞作为效应细胞，对肌肉注射巴塔病毒疫苗的猪血清进行ADCC滴定，并将结果与病毒中和试验的结果进行比较。事实证明，ADCC是一种更为灵敏的技术，因此可能为可靠的诊断试验提供基础。发现从血液和腹腔渗出液中分离得到的部分纯化淋巴细胞、多形核白细胞以及渗出液中的巨噬细胞可通过超免疫血清介导ADCC，但在它们细胞毒性作用的效率和时间方面观察到了差异。
