Perrin L H, Zinkernagel R M, Oldstone M B
J Exp Med. 1977 Oct 1;146(4):949-69. doi: 10.1084/jem.146.4.949.
After vaccinia virus vaccination of human volunteers, local indurations developed within 10 days, and regional adenopathy was detected in half of the individuals. Their peripheral blood lymphocytes (PBL) harvested at different days after vaccination showed specific activity against target cells infected with vaccinia virus with a peak activity at day 7. The specificity of the cytotoxic activity was not related to HLA markers, since autologous, homologous, and heterologous infected target cells were lysed with the same efficiency. The cytotoxic activity was caused by PBL that did not rosette with sheep erythrocytes and could be depleted by more than 90 percent by removing Fc receptor-bearing cells. T-cell- depleted PBL showed a one-half to two times greater cytotoxicity than intact PBL. The cytotoxic activity could also be abrogated by more than 95 percent by rabbit Fab(2) anti-human IgG. On the other hand, nonimmune PBL lysed vaccinia-infected target cells in the presence of specific antibodies against vaccinia virus, thus demonstrating that ADCC could be efficient in lysing vaccinia-infected target cells. We conclude that after vaccination, antibody-forming cells arise and provide specific anti-viral antibody and that the cytotoxic cells detected in this reaction are K cells. These experiments suggest that antibody-dependent cell cytotoxicity may be of major importance in the recovery of man to virus infections.
在给人类志愿者接种牛痘病毒后，10天内出现局部硬结，半数个体检测到局部淋巴结病。在接种后不同时间采集的外周血淋巴细胞（PBL）对感染牛痘病毒的靶细胞表现出特异性活性，在第7天活性达到峰值。细胞毒性活性的特异性与HLA标记无关，因为自体、同源和异源感染的靶细胞以相同效率被裂解。细胞毒性活性由不与绵羊红细胞形成玫瑰花结的PBL引起，通过去除携带Fc受体的细胞可使其减少90%以上。去除T细胞的PBL比完整的PBL表现出高一半到两倍的细胞毒性。细胞毒性活性也可被兔Fab(2)抗人IgG消除95%以上。另一方面，非免疫PBL在存在抗牛痘病毒特异性抗体的情况下可裂解感染牛痘的靶细胞，从而证明抗体依赖性细胞毒性在裂解感染牛痘的靶细胞中可能有效。我们得出结论，接种后产生抗体形成细胞并提供特异性抗病毒抗体，并且在该反应中检测到的细胞毒性细胞是K细胞。这些实验表明抗体依赖性细胞毒性在人类从病毒感染中恢复可能起主要作用。
