van den Heuvel Sander, Kipreos Edward T
Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Methods Cell Biol. 2012;107:265-94. doi: 10.1016/B978-0-12-394620-1.00009-6.
Caenorhabditis elegans is an important system for the study of cell cycle regulation in the context of animal development. One of the most powerful features of C. elegans is the invariant cell lineage in which somatic cells initiate cell division at specific times within the developmental program. The cell lineage is fully known and provides the foundation for the analysis of cell cycle progression at single-cell resolution in a multicellular organism. In this chapter, we describe the different types of cell cycles observed in C. elegans, and provide methods and reagents for the analysis of cell cycle progression as well as specific cell cycle phases. We also provide strategies for the analysis and proper interpretation of cell cycle and checkpoint mutants.
秀丽隐杆线虫是在动物发育背景下研究细胞周期调控的重要系统。秀丽隐杆线虫最强大的特征之一是其恒定不变的细胞谱系，其中体细胞在发育程序内的特定时间启动细胞分裂。细胞谱系已完全明确，为在多细胞生物体中以单细胞分辨率分析细胞周期进程奠定了基础。在本章中，我们描述了秀丽隐杆线虫中观察到的不同类型的细胞周期，并提供了分析细胞周期进程以及特定细胞周期阶段的方法和试剂。我们还提供了分析和正确解释细胞周期及检查点突变体的策略。
