Laboratório Nacional de Computação Científica, Petrópolis, Brazil.
Infect Genet Evol. 2012 Mar;12(2):309-14. doi: 10.1016/j.meegid.2011.12.011. Epub 2011 Dec 27.
Evolutionary studies on dengue virus have frequently focused on intra-serotype diversity or on specific epidemics. In this study, we compiled a comprehensive data set of the envelope gene of dengue virus serotypes and conducted an extensive comparative study of evolutionary molecular epidemiology. We found that substitution rates are homogeneous among dengue serotypes, although their population dynamics have differed over the past few years as inferred by Bayesian coalescent methods. On a global scale, DENV-2 is the serotype with the highest effective population size. The genealogies also showed geographical structure within the serotypes. Finally, we also explored the causes of dengue virus serotype diversification by investigating the plausibility that it was driven by adaptive changes. Our results suggest that the envelope gene is under significant purifying selection and the hypothesis that dengue virus serotype diversification was the result of stochastic events cannot be ruled out.
登革热病毒的进化研究通常集中在同型内多样性或特定的流行上。在这项研究中，我们编译了登革热病毒血清型包膜基因的综合数据集，并对进化分子流行病学进行了广泛的比较研究。我们发现，尽管过去几年间登革热血清型的种群动态有所不同，但替代率在血清型之间是均匀的，这是由贝叶斯合并方法推断的。在全球范围内，DENV-2 是有效种群规模最大的血清型。系统发育还显示了血清型内的地理结构。最后，我们还通过调查适应性变化是否是导致登革热病毒血清型多样化的原因来探索其原因。我们的结果表明，包膜基因受到显著的纯化选择，不能排除登革热病毒血清型多样化是随机事件的结果这一假设。
