5-氨基-2-巯基-1,3,4-噻二唑修饰的一次性电化学 DNA 分析传感器。
5-amino-2-mercapto-1,3,4-thidiazole modified single-use sensors for electrochemical DNA analysis.
机构信息
Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, Izmir, Turkey.
出版信息
Colloids Surf B Biointerfaces. 2012 May 1;93:116-20. doi: 10.1016/j.colsurfb.2011.12.021. Epub 2011 Dec 27.
In this study, single-use graphite electrodes modified with 5-amino-2-mercapto-1,3,4-thidiazole (AMT) were fabricated for electrochemical monitoring of DNA. The surfaces of AMT modified pencil graphite electrodes (PGEs) were characterized by scanning electron microscopy (SEM). Electrochemical behaviors of these electrodes were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The effects of pretreatment of PGE and immobilization time and concentration of AMT were studied to facilitate an effective immobilization on PGE surface. The efficiency of immobilization is verified by measuring the oxidation signals of either AMT itself or adenine base of DNA, immobilized onto AMT modified electrodes.
在这项研究中，制备了经过 5-氨基-2-巯基-1,3,4-噻二唑（AMT）修饰的一次性使用石墨电极，用于 DNA 的电化学监测。通过扫描电子显微镜（SEM）对 AMT 修饰的铅笔石墨电极（PGE）的表面进行了表征。使用差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）研究了这些电极的电化学行为。研究了 PGE 的预处理、AMT 的固定时间和浓度对其在 PGE 表面有效固定的影响。通过测量固定在 AMT 修饰电极上的 AMT 本身或 DNA 的腺嘌呤碱基的氧化信号，验证了固定的效率。
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