Department of Mechanical Engineering, Vanderbilt University, Nashville, Tennessee 37235-1592, USA.
Anal Chem. 2012 Feb 21;84(4):2017-24. doi: 10.1021/ac203212g. Epub 2012 Feb 1.
Through integration of a MOSFET-based microfluidic Coulter counter with a dc-dielectrophoretic cell sorter, we demonstrate simultaneous on-chip cell separation and sizing with three different samples including 1) binary mixtures of polystyrene beads, 2) yeast cells of continuous size distribution, and 3) mixtures of 4T1 tumor cells and murine bone marrow cells. For cells with continuous size distribution, it is found that the receiver operator characteristic analysis is an ideal method to characterize the separation performance. The characterization results indicate that dc-DEP separation performance degrades as the sorting throughput (cell sorting rate) increases, which provides insights into the design and operation of size-based microfluidic cell separation.
通过将基于 MOSFET 的微流控库尔特计数器与直流介电泳细胞分选器集成，我们展示了同时对三种不同样品进行芯片上细胞分离和尺寸分析的功能，这三种样品分别为：1）聚苯乙烯珠的二元混合物，2）具有连续尺寸分布的酵母细胞，和 3）4T1 肿瘤细胞和鼠骨髓细胞的混合物。对于具有连续尺寸分布的细胞，发现接收者操作特征分析是一种表征分离性能的理想方法。表征结果表明，直流介电泳分离性能随着分选通量（细胞分选率）的增加而降低，这为基于尺寸的微流控细胞分离的设计和操作提供了思路。
