α-硫辛酸对小鼠分离的原始卵泡发育能力的影响。
The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles.
机构信息
School of Biology, Damghan University, Damghan, Iran.
出版信息
J Assist Reprod Genet. 2012 Feb;29(2):175-83. doi: 10.1007/s10815-011-9706-6. Epub 2012 Jan 10.
Abstract
PURPOSE
This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue.
METHODS
Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α- minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively.
RESULTS
In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p < 0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p < 0.05).
CONCLUSION
ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.
摘要
目的
本研究旨在探讨α-硫辛酸（ALA）对培养的来自小鼠卵巢组织的原始卵泡的活性氧（ROS）产生、总抗氧化能力（TAC）和发育能力的影响。
方法
从未成熟的小鼠卵巢中分离出原始卵泡，并在α-最小必需培养基中培养，培养基中添加不同浓度（0、50、100、250 和 500 μM）的 ALA。评估卵泡生长、卵母细胞成熟和胚胎发育。另外，用分光光度法分别在培养 0、24、48、72 和 96 小时后测量 ROS 和 TAC，用铁还原/抗氧化能力（FRAP）测定法测量 TAC。
结果
在存在 100 μM ALA 的情况下，卵泡、卵母细胞和胚胎的发育率明显高于其他组（p < 0.05）。与对照组相比，培养 96 小时后，ALA 组 ROS 减少，TAC 增加（p < 0.05）。
结论
ALA（100 μM）可提高卵泡的体外发育。这种作用可能是通过在培养期间降低 ROS 浓度和增加卵泡 TAC 水平来介导的。
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