Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
Vet Comp Oncol. 2013 Mar;11(1):30-50. doi: 10.1111/j.1476-5829.2011.00299.x. Epub 2011 Nov 23.
Molecular characterization of tumour cell lines is increasingly regarded as a prerequisite for defining their validity as models of in vivo neoplasia. We present the first comprehensive catalogue of genomic and transcriptional characteristics of five widely used canine lymphoid tumour cell lines. High-resolution microarray-based comparative genomic hybridization defined their unique profiles of genomic DNA copy number imbalance. Multicolour fluorescence in situ hybridization identified aberrant gains of MYC, KIT and FLT3 and deletions of PTEN and CDKN2 in individual cell lines, and also revealed examples of extensive structural chromosome reorganization. Gene expression profiling and RT-PCR analyses defined the relationship between genomic imbalance and transcriptional dysregulation in each cell line, clarifying their relevance as models of discrete functional pathways with biological and therapeutic significance. In combination, these data provide an extensive resource of molecular data for directing the appropriate use of these cell lines as tools for studying canine lymphoid neoplasia.
肿瘤细胞系的分子特征越来越被认为是确定其作为体内肿瘤模型有效性的前提条件。我们首次全面描述了五种广泛使用的犬淋巴样肿瘤细胞系的基因组和转录特征。基于高分辨率微阵列的比较基因组杂交定义了它们独特的基因组 DNA 拷贝数失衡谱。多色荧光原位杂交鉴定了单个细胞系中 MYC、KIT 和 FLT3 的异常获得以及 PTEN 和 CDKN2 的缺失，并揭示了广泛的结构染色体重排的例子。基因表达谱和 RT-PCR 分析定义了每个细胞系中基因组失衡与转录失调之间的关系，阐明了它们作为具有生物学和治疗意义的离散功能途径模型的相关性。总之，这些数据为指导这些细胞系作为研究犬淋巴样肿瘤的工具的合理使用提供了广泛的分子数据资源。
