Department of Food and Environmental Sciences, P.O. Box 27 (Latokartanonkaari 11), 00014 University of Helsinki, Finland.
Bioresour Technol. 2012 Mar;107:275-81. doi: 10.1016/j.biortech.2011.12.101. Epub 2011 Dec 27.
Pectinolytic enzymes, steam explosion and alkaline treatment were used to assess the role of pectin for the accessibility of hydrolytic enzymes in the enzymatic hydrolysis of biomass. Hemp (Cannabis sativa L.), a potential energy crop especially in boreal climate with a low need of fertilizers, was used in the study either as untreated or anaerobically preserved raw material. Addition of pectinases increased the hydrolysis yield by 26%, 54%, and 64% from the theoretical carbohydrates of untreated, acid, and alkali-preserved materials, respectively. Steam explosion and hot alkali treatment increased the conversion of the total carbohydrates by 78% and 60%, respectively, compared to the untreated hemp. Elevated separation of cells within the hemp stalk tissues and an increased surface area was revealed after hot alkali or pectinase treatments, contributing to the increased conversion to sugars by commercial enzymes.
果胶酶、蒸汽爆破和碱处理被用于评估果胶对水解酶在生物质酶解中可及性的作用。麻（Cannabis sativa L.）是一种有潜力的能源作物，特别是在肥料需求较低的北方气候条件下，本研究使用未经处理或厌氧保存的原料。与未经处理、酸处理和碱处理的材料的理论碳水化合物相比，添加果胶酶分别将水解产率提高了 26%、54%和 64%。与未经处理的麻相比，蒸汽爆破和热碱处理分别将总碳水化合物的转化率提高了 78%和 60%。热碱或果胶酶处理后，麻秆组织内细胞的分离度增加，表面积增加，这有助于提高商业酶对糖的转化率。
