Laboratoire des Sciences de l'Ingénieur pour l'Environnement, LaSIE, UMR CNRS 7356, Université de La Rochelle, Avenue Michel Crépeau, 17042 La Rochelle, France; Laboratoire de Chimie de la Matière Condensée, Research Team: Procédés pour l'Energie et l'Environnement, Faculté Polydisciplinaire de Taza, Université Sidi Mohamed Ben Abdellah, Morocco.
Equipe Approches Moléculaires Environnement-Santé, UMR CNRS 7266, LIENSs, Université de La Rochelle, France.
Bioresour Technol. 2018 Jun;257:192-200. doi: 10.1016/j.biortech.2018.02.107. Epub 2018 Feb 23.
The aim of this study was to investigate a pretreatment by combined HSO acid-catalyst and thermomechanical process to improve hemicelluloses solubilization of hemp hurds and subsequently enzymatic hydrolysis extent of potentially fermentable sugars. It was found that the sugars released were gradually increased with treatment severity. Soluble sugars generated before enzymatic hydrolysis (R) increased up to 2.23 g/L indicating that autohydrolysis reaction occurred during pretreatment. Consequently, the solubilization of hemicelluloses was correlated with combined severity factor (CS). As a result, increase of overall reducing sugars (ORS) from 23.4% (untreated) to 81.4% was observed at optimized conditions of steaming temperature of 165 °C for 30 min and acid loading of 62.9 g/kg DM (dry material) corresponding to CS = 1.2, with limited production of identified by-products: 0.035 g/L and 0.46 g/L (per 100 g DM) for furfural and HMF, respectively. Structural and physicochemical modifications of biomass were observed by FTIR, A and SEM.
本研究旨在探讨一种预处理方法，即联合使用 HSO 酸催化剂和热机械过程，以提高麻屑中半纤维素的溶解性能，进而提高潜在可发酵糖的酶解程度。研究发现，随着处理强度的增加，释放的糖逐渐增加。在酶解之前产生的可溶性糖（R）增加到 2.23g/L，表明在预处理过程中发生了自水解反应。因此，半纤维素的溶解性能与联合严重度因子（CS）有关。结果表明，在优化条件下（蒸汽温度为 165°C，时间为 30min，酸用量为 62.9g/kgDM），总还原糖（ORS）从未经处理的 23.4%增加到 81.4%，相应的 CS 值为 1.2，同时产生的鉴定副产物的量有限：糠醛和 5-羟甲基糠醛的产量分别为 0.035g/L 和 0.46g/L（每 100gDM）。通过 FTIR、A 和 SEM 观察到生物质的结构和物理化学改性。
