Departamento de Química, Universidade Federal de São Carlos, CEP 13.565-905, CP 676, São Carlos, SP, Brazil.
Nat Prod Res. 2013;27(1):9-16. doi: 10.1080/14786419.2011.647021. Epub 2012 Jan 12.
In our continuous studies on the chemistry of the endophytic fungus Penicillium griseoroseum, an endophyte isolated from fruits of Coffea arabica, we isolated clavatol, a dimethylated tetraketide, and its dimer which appears to be a novel natural compound. The studies also resulted in the identification of two known tetronic acids, viridicatic acid and terrestric acid, found in ethyl acetate and n-butanol extracts. Spectroscopic studies using 1-D and 2-D NMR and MS/MS analysis were performed to determine the structures of these compounds, first reported by this Penicillium. Two other tetronic acids congeners were identified through HPLC/MS/MS studies, based on fragmentation pattern of ions produced from ionised tetronic acids, and UV light absorptions.
在对内生真菌青霉（Penicillium griseoroseum）的化学物质的持续研究中，我们从咖啡果实中分离出这种内生真菌，从中分离出克拉维醇，这是一种二甲基四酮，以及它的二聚体，这似乎是一种新的天然化合物。这些研究还鉴定出两种已知的四酮酸，即绿蝇酸和地衣酸，它们存在于乙酸乙酯和正丁醇提取物中。通过使用 1-D 和 2-D NMR 和 MS/MS 分析进行的光谱研究，确定了这些化合物的结构，这些化合物是由这种青霉首次报告的。通过 HPLC/MS/MS 研究，根据从离子化四酮酸产生的离子的碎片模式和紫外光吸收，鉴定出另外两种四酮酸同系物。
