Humboldt-Universität zu Berlin, Department of Chemistry, Laboratory for Organic Synthesis of Functional Systems, Brook-Taylor-Strasse 2, D-12489 Berlin, Germany.
J Am Chem Soc. 2012 Feb 1;134(4):2385-91. doi: 10.1021/ja2104944. Epub 2012 Jan 20.
The peptide-mediated functionalization of inorganic particle surfaces is demonstrated on gadolinium oxide (GdO) particles, revealing specific means to functionalize nano- or microparticles. Phage display screening is exploited to select 12mer peptides, which exhibit sequence-specific adhesion onto surfaces of GdO particles. These peptide adhesion domains are exploited to effectively decorate GdO particles with fluorescently labeled poly(ethylene oxide) (PEO), proving to result in a stable surface modification as shown by significant reduction of protein adsorption by 80%, compared to nonfunctionalized particles. Peptide adhesion and stability of the noncovalent coating are investigated by adsorption/elution experiments and Langmuir isotherms. Fluorescence microscopy, contact angle, and energy dispersive X-ray (EDX) measurements confirmed the sequence specificity of the interactions by comparing adhesion sequences with scrambled peptide sequences. Noncovalent, but specific modification of inorganic particle surfaces represents a generic strategy to modulate functionality and function of nano- or microparticle surfaces.
本文展示了在氧化钆（GdO）颗粒上通过肽介导的功能化无机颗粒表面，揭示了功能化纳米或微米颗粒的特定方法。利用噬菌体展示筛选技术选择了 12 个展示序列特异性黏附到 GdO 颗粒表面的肽。这些肽黏附结构域可有效地将荧光标记的聚环氧乙烷（PEO）修饰到 GdO 颗粒上，与非功能化颗粒相比，通过显著减少 80%的蛋白质吸附，证明了其稳定的表面修饰效果。通过吸附/洗脱实验和朗缪尔等温线研究了肽黏附和非共价涂层的稳定性。荧光显微镜、接触角和能量色散 X 射线（EDX）测量通过比较黏附序列与乱序肽序列证实了相互作用的序列特异性。非共价但特定的无机颗粒表面修饰代表了一种调节纳米或微米颗粒表面功能和功能的通用策略。
