仿生偶联反应驱动的膜组装。
Membrane assembly driven by a biomimetic coupling reaction.
机构信息
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
出版信息
J Am Chem Soc. 2012 Jan 18;134(2):751-3. doi: 10.1021/ja2076873. Epub 2011 Dec 29.
Abstract
One of the major goals of synthetic biology is the development of non-natural cellular systems. In this work, we describe a catalytic biomimetic coupling reaction capable of driving the de novo self-assembly of phospholipid membranes. Our system features a coppercatalyzed azide-alkyne cycloaddition that results in the formation of a triazole-containing phospholipid analogue. Concomitant assembly of membranes occurs spontaneously, not requiring preexisting membranes to house catalysts or precursors. The substitution of efficient synthetic reactions for key biochemical processes may offer a general route toward synthetic biological systems.
摘要
合成生物学的主要目标之一是开发非天然的细胞系统。在这项工作中，我们描述了一种催化仿生偶联反应，能够驱动磷脂膜的从头自组装。我们的系统具有铜催化的叠氮-炔环加成反应，导致形成含有三唑的磷脂类似物。膜的伴随组装自发发生，不需要预先存在的膜来容纳催化剂或前体。用有效的合成反应替代关键的生化过程可能为合成生物学系统提供一条通用途径。
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