Pol II(G)介导的转录调控以及 Gdown1 和 TFIIF 与 Pol II 的竞争相互作用。
Transcriptional regulation by Pol II(G) involving mediator and competitive interactions of Gdown1 and TFIIF with Pol II.
机构信息
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA.
出版信息
Mol Cell. 2012 Jan 13;45(1):51-63. doi: 10.1016/j.molcel.2011.12.014.
Abstract
Pol II(G) is a distinct form of RNA polymerase II that contains the tightly associated Gdown1 polypeptide (encoded by POLR2M). Unlike Pol II, Pol II(G) is highly dependent upon Mediator for robust activator-dependent transcription in a biochemically defined in vitro system. Here, in vitro studies show that Gdown1 competes with TFIIF for binding to the RPB1 and RPB5 subunits of Pol II, thereby inhibiting an essential function of TFIIF in preinitiation complex assembly, but also that Mediator can actually facilitate Pol II(G) binding to the promoter prior to subsequent Mediator functions. Complementary ChIP and RNAi analyses reveal that Pol II(G) is recruited to promoter regions of subsets of actively transcribed genes, where it appears to restrict transcription. These and other results suggest that Pol II(G) may act to modulate some genes while simultaneously, as a poised (noninitiated) polymerase, setting the stage for Mediator-dependent enhancement of their activity.
摘要
Pol II(G) 是一种特殊的 RNA 聚合酶 II，它包含紧密相关的 Gdown1 多肽（由 POLR2M 编码）。与 Pol II 不同，Pol II(G) 在生化定义的体外系统中高度依赖 Mediator 才能进行强有力的激活剂依赖性转录。在这里，体外研究表明 Gdown1 与 TFIIF 竞争结合到 Pol II 的 RPB1 和 RPB5 亚基上，从而抑制 TFIIF 在起始前复合物组装中的一个基本功能，但 Mediator 实际上可以促进 Pol II(G) 在随后的 Mediator 功能之前结合到启动子上。互补 ChIP 和 RNAi 分析表明，Pol II(G) 被招募到活跃转录基因的启动子区域，在那里它似乎限制了转录。这些和其他结果表明，Pol II(G) 可能会作用于一些基因，同时作为一种处于静止（未起始）状态的聚合酶，为 Mediator 依赖性增强其活性奠定基础。
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