Laboratory of Biochemistry and Molecular Biology, Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.
Nat Rev Genet. 2010 Nov;11(11):761-72. doi: 10.1038/nrg2901. Epub 2010 Oct 13.
The Mediator is an evolutionarily conserved, multiprotein complex that is a key regulator of protein-coding genes. In metazoan cells, multiple pathways that are responsible for homeostasis, cell growth and differentiation converge on the Mediator through transcriptional activators and repressors that target one or more of the almost 30 subunits of this complex. Besides interacting directly with RNA polymerase II, Mediator has multiple functions and can interact with and coordinate the action of numerous other co-activators and co-repressors, including those acting at the level of chromatin. These interactions ultimately allow the Mediator to deliver outputs that range from maximal activation of genes to modulation of basal transcription to long-term epigenetic silencing.
中介体是一个进化上保守的、多蛋白复合物，是蛋白质编码基因的关键调节因子。在后生动物细胞中，负责维持体内平衡、细胞生长和分化的多条途径通过转录激活因子和抑制因子汇聚到中介体上，这些转录激活因子和抑制因子靶向该复合物的几乎 30 个亚基中的一个或多个。除了与 RNA 聚合酶 II 直接相互作用外，中介体还具有多种功能，可以与众多其他共激活因子和共抑制因子相互作用并协调它们的作用，包括那些在染色质水平上发挥作用的因子。这些相互作用最终使中介体能够传递从基因的最大激活到基础转录的调节再到长期表观遗传沉默的各种输出。
