Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.
Methods. 2012 Jul;57(3):383-91. doi: 10.1016/j.ymeth.2012.01.001. Epub 2012 Jan 10.
Highly effective vaccines have yet to be identified for many widespread infectious diseases including HIV, tuberculosis and malaria. Many vaccine candidates for these diseases have been designed to induce both cellular and humoral immunity, and measurement of the induced cellular immune response and antibody response is critical for monitoring immunogenicity. The flow cytometric intracellular cytokine staining assay is one of the primary assays for enumerating vaccine-induced T cells in vaccine clinical trials. The assay is flexible, allowing for measurement of various cytokines or functions and phenotyping markers, and the assay can be validated. Changes in other cell types such as innate immune cells are monitored by flow cytometric phenotyping assays. Cell sorting of vaccine-induced T cells and B cells is used to allow genomic and transcriptional analysis of these cells. Thus, flow cytometric methods are commonly used in trials testing the next generation of vaccines.
目前，仍有许多广泛流行的传染病（包括艾滋病毒、结核病和疟疾）没有有效的疫苗。这些疾病的许多候选疫苗旨在诱导细胞和体液免疫，而诱导的细胞免疫应答和抗体应答的测量对于监测免疫原性至关重要。流式细胞术细胞内细胞因子染色分析是疫苗临床试验中计数疫苗诱导 T 细胞的主要分析方法之一。该分析方法灵活，可用于测量各种细胞因子或功能和表型标志物，并且可以进行验证。通过流式细胞术表型分析来监测其他细胞类型（如固有免疫细胞）的变化。疫苗诱导的 T 细胞和 B 细胞的细胞分选用于允许对这些细胞进行基因组和转录组分析。因此，流式细胞术方法常用于下一代疫苗的临床试验。
