Nukavarapu Syam P, Amini Ami R
Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:2464-7. doi: 10.1109/IEMBS.2011.6090684.
Bone tissue engineering offers perhaps the most attractive treatment option for bone repair/regeneration as it eliminates complications of other bone grafting options (i.e., availability and immunogenicity issues of autografts and allografts, respectively). However, scaffold-based bone tissue engineering is largely limited by inadequate vascaularization, and as a result, bone formation is often restricted to the construct's periphery. In this study, we offer a two-pronged approach to overcome periphery-limited bone and vascular formation. We have developed optimally designed, mechanically strong, biodegradable scaffolds with increased porosity and interconnectivity. We have also identified and isolated superior, clinically-relevant cell populations (peripheral blood-derived endothelial progenitor cells (EPCs), and bone marrow-derived mesenchymal stem cells (MSCs)). In combination, we have developed a synthetic graft system suitable for the regeneration of vascularized bone.
骨组织工程为骨修复/再生提供了可能最具吸引力的治疗选择，因为它消除了其他骨移植选择的并发症（即分别为自体骨和同种异体骨的可用性和免疫原性问题）。然而基于支架的骨组织工程在很大程度上受到血管化不足的限制，因此，骨形成通常局限于构建体的周边。在本研究中，我们提供了一种双管齐下的方法来克服周边受限的骨和血管形成。我们开发了设计优化、机械强度高、具有更高孔隙率和连通性的可生物降解支架。我们还鉴定并分离出了优质的、与临床相关的细胞群体（外周血来源的内皮祖细胞（EPCs）和骨髓来源的间充质干细胞（MSCs））。综合起来，我们开发了一种适用于血管化骨再生的合成移植系统。
