Section Sciences of Vision, Department of Medicine and Ageing Sciences, University G. D'Annunzio, Chieti, Italy.
Ophthalmologica. 2012;227(3):139-45. doi: 10.1159/000334967. Epub 2012 Jan 19.
To assess the reproducibility and repeatability of peripapillary retinal nerve fibre layer (RNFL) thickness measurements using a spectral-domain optical coherence tomography (SD-OCT) device in healthy subjects.
In this observational study, 68 young Caucasian healthy volunteers (68 eyes) were subjected to Cirrus™ high-definition (HD) OCT (Zeiss) peripapillary RNFL thickness measurements by two experienced examiners in two different sessions. Average, 4-quadrant and 12-clock-hour sector RNFL thicknesses were analysed. For each option, intra-observer, intrasession repeatability and interobserver, intersession reproducibility were tested. To assess the repeatability of measurements, the Bland and Altman plots were used and the coefficient of repeatability was calculated. Interobserver and intersession reproducibilities were analysed by means of concordance correlation coefficients (CCCs).
The sample age ranged from 21 to 39 years (mean 29.09, standard deviation ±5.21). The average RNFL thickness ranged from 90.97 to 91.46 and from 91.34 to 91.78 μm, for the first and the second operator, respectively. The highest repeatability and reproducibility were obtained for average RNFL thickness with coefficients of repeatability of 5.30 and 6.05 μm for the first and the second operator, interoperator CCCs of 0.95 and 0.96 for the first and the second session, and intersession CCCs of 0.96 and 0.97 for the first and the second operator, respectively.
Cirrus OCT peripapillary average RNFL thickness measurement in young healthy subjects showed high interoperator and intersession reproducibility. Intrasession repeatability as tested by coefficient of repeatability was next to the device resolution, with very similar results between the two operators. When analysing quadrant and clock hour sector RNFL thickness measurements, both repeatability and reproducibility tend to decrease.
评估使用频域光学相干断层扫描（SD-OCT）设备在健康受试者中测量视盘周围视网膜神经纤维层（RNFL）厚度的可重复性和可重复性。
在这项观察性研究中，对 68 名年轻的白种人健康志愿者（68 只眼）进行了 Cirrus™高清（HD）OCT（蔡司）视盘周围 RNFL 厚度测量，由两名经验丰富的检查者在两次不同的检查中进行。分析了平均、4 象限和 12 时钟小时扇形 RNFL 厚度。对于每个选项，测试了观察者内、单次检查内的重复性和观察者间、两次检查间的再现性。为了评估测量的可重复性，使用了 Bland 和 Altman 图并计算了重复性系数。通过一致性相关系数（CCC）分析了观察者间和检查间的再现性。
样本年龄范围为 21 至 39 岁（平均 29.09，标准差±5.21）。第一位和第二位操作者测量的平均 RNFL 厚度分别为 90.97 至 91.46 和 91.34 至 91.78μm。重复性和再现性最高的是平均 RNFL 厚度，第一位和第二位操作者的重复性系数分别为 5.30 和 6.05μm，第一位和第二位检查者的观察者间 CCC 分别为 0.95 和 0.96，第一位和第二位检查者的检查间 CCC 分别为 0.96 和 0.97。
在年轻健康受试者中，Cirrus OCT 视盘周围平均 RNFL 厚度测量具有较高的观察者间和检查间可重复性。以重复性系数测试的单次检查内重复性接近设备分辨率，两位操作者的结果非常相似。在分析象限和时钟小时扇形 RNFL 厚度测量时，重复性和再现性均有下降趋势。
