Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, the Netherlands.
Appl Microbiol Biotechnol. 2012 Nov;96(3):729-37. doi: 10.1007/s00253-012-3871-0. Epub 2012 Jan 20.
The major murein and pseudomurein cell wall-binding domains, i.e., the Lysin Motif (LysM) (Pfam PF01476) and pseudomurein cell wall-binding (PMB) (Pfam PF09373) motif, respectively, were genetically fused. The fusion protein is capable of binding to both murein- and pseudomurein-containing cell walls. In addition, it also binds to chitin, the major polymer of fungal cell walls. Binding is influenced by pH and occurs at a pH close to the pI of the binding protein. Functional studies on truncated versions of the fusion protein revealed that murein and chitin binding is provided by the LysM domain, while binding to pseudomurein is achieved through the PMB domain.
主要的肽聚糖和假肽聚糖细胞壁结合结构域，即赖氨酸基序（LysM）（Pfam PF01476）和假肽聚糖细胞壁结合（PMB）（Pfam PF09373）基序，分别进行了基因融合。融合蛋白能够与含有肽聚糖和假肽聚糖的细胞壁结合。此外，它还与真菌细胞壁的主要聚合物几丁质结合。结合受 pH 值影响，发生在接近结合蛋白等电点的 pH 值附近。融合蛋白截短版本的功能研究表明，肽聚糖和几丁质的结合由 LysM 结构域提供，而与假肽聚糖的结合则通过 PMB 结构域实现。
