Yang Jing, Zhao Na, Su Nai-Lun, Sun Jian-Lan, Lv Tie-Gang, Chen Zong-Bo
Pediatric Department of the Affiliated Hospital of Qingdao University Medical School, Qingdao, PR ChinaQingdao, PR China.
Scand J Infect Dis. 2012 Jun;44(6):465-9. doi: 10.3109/00365548.2011.649490. Epub 2012 Jan 21.
Enterovirus 71 (EV71) is one of the common causative agents of hand, foot and mouth disease (HFMD), and is associated with several outbreaks with neurological complications including encephalitis. This study investigated the polymorphisms of interferon gamma (IFN-γ)+874 T/A and interleukin 10 (IL-10)-1082 G/A in 65 Chinese patients with EV71 encephalitis and 113 Chinese HFMD patients without complications. The polymorphisms of IFN-γ+874 T/A and IL-10-1082 G/A were determined by polymerase chain reaction (PCR)-amplification refractory mutation system (ARMS) and PCR-sequence-specific primer (SSP) analysis, respectively. The IFN-γ + 874 A allele was observed with significantly greater frequency in patients with EV71 encephalitis (76.2%) compared with HFMD patients without complications (61.1%, p < 0.01). Similarly, the IL-10 - 1082 A allele was observed with significantly greater frequency in patients with EV71 encephalitis (86.2%) compared with HFMD patients without complications (77.0%, p < 0.05). IFN-γ + 874 A and IL-10 - 1082 A alleles are associated with susceptibility to EV71 encephalitis in Chinese patients.
肠道病毒71型（EV71）是手足口病（HFMD）常见的病原体之一，与包括脑炎在内的几起伴有神经并发症的疫情有关。本研究调查了65例中国EV71脑炎患者和113例无并发症的中国手足口病患者中干扰素γ（IFN-γ）+874 T/A和白细胞介素10（IL-10）-1082 G/A的多态性。IFN-γ+874 T/A和IL-10-1082 G/A的多态性分别通过聚合酶链反应（PCR）-扩增阻滞突变系统（ARMS）和PCR-序列特异性引物（SSP）分析来确定。与无并发症的手足口病患者（61.1%，p<0.01）相比，EV71脑炎患者中IFN-γ+874 A等位基因的出现频率显著更高（76.2%）。同样，与无并发症的手足口病患者（77.0%，p<0.05）相比，EV71脑炎患者中IL-10 - 1082 A等位基因的出现频率显著更高（86.2%）。IFN-γ + 874 A和IL-10 - 1082 A等位基因与中国患者对EV71脑炎的易感性有关。
