From the 1 Infectious Diseases Department, Qilu Children's Hospital of Shandong University , Jinan , PR China.
Infect Dis (Lond). 2015 Jun;47(6):418-22. doi: 10.3109/00365548.2015.1007473. Epub 2015 Mar 9.
The study was performed in 36 Chinese patients with Enterovirus 71 (EV71) encephalitis and 141 patients with EV71-related hand, foot and mouth disease (HFMD) without encephalitis. Genotyping was determined by polymerase chain reaction- restriction fragment length polymorphism. Patients with EV71 encephalitis had a significantly higher frequency of interleukin-8 (IL-8)-251TT genotype than patients with EV71-related HFMD without encephalitis (55.6% vs 31.2%, p = 0.023). The frequency of IL-8-251T alleles was significantly higher among patients with EV71 encephalitis than in patients with EV71-related HFMD without encephalitis (72.2% vs 58.9%, odds ratio 1.8, 95% confidence interval 1.0-3.2, p = 0.038). There were significant differences in gender, age, fever days, white blood cell count, C-reactive protein and blood glucose concentration and IL-8 levels among genotypes of IL-8-251A/T in EV71-infected patients, but no significant differences in alanine or aspartate aminotransferase, creatine kinase-myocardial isozyme and cerebrospinal fluid in patients with EV71 encephalitis. These findings suggest that the IL-8-251T allele is associated with susceptibility to EV71 encephalitis in Chinese patients.
该研究纳入了 36 例肠道病毒 71（EV71）脑炎患儿和 141 例无脑炎的 EV71 相关手足口病（HFMD）患儿。通过聚合酶链反应-限制性片段长度多态性确定基因型。EV71 脑炎患儿白细胞介素-8（IL-8）-251TT 基因型的频率显著高于无脑炎的 EV71 相关 HFMD 患儿（55.6% vs 31.2%，p=0.023）。EV71 脑炎患儿 IL-8-251T 等位基因的频率显著高于无脑炎的 EV71 相关 HFMD 患儿（72.2% vs 58.9%，比值比 1.8，95%置信区间 1.0-3.2，p=0.038）。在 EV71 感染患儿中，IL-8-251A/T 基因型之间的性别、年龄、发热天数、白细胞计数、C 反应蛋白和血糖浓度以及 IL-8 水平存在显著差异，但 EV71 脑炎患儿的丙氨酸或天冬氨酸氨基转移酶、肌酸激酶同工酶和脑脊液无显著差异。这些发现提示 IL-8-251T 等位基因与中国患儿易患 EV71 脑炎相关。
