Pediatric Department, Affiliated Hospital of Qingdao University Medical School, 16 Jangsu road, Qingdao, 266100, Shandong, China.
Inflammation. 2013 Aug;36(4):977-81. doi: 10.1007/s10753-013-9629-8.
Enterovirus 71 (EV71) is one of the common pathogenic agents of hand, foot, and mouth disease (HFMD) and is associated with severe complications including encephalitis. Interleukin (IL)-17F plays an important role in tissue inflammation by inducing release of proinflammatory cytokines and chemokines. We investigated the association between EV71 encephalitis and of IL-17F 7488T/C (rs763780) gene polymorphism, which is known to cause a His-to-Arg substitution at amino acid 161. The study was performed in 58 Chinese patients with EV71 encephalitis and 127 Chinese patients with EV71-related HFMD without complications. Genotyping was determined by the polymerase chain reaction-restriction fragment length polymorphism technique. The patients with EV71 encephalitis had a significantly lower frequency of the IL-17F 7488TC+CC genotypes (10.3%) as compared to the patients with EV71-related HFMD without complications (27.6%, p = 0.008). The frequency of IL-17F 7488C alleles was also significantly lower among the patients with EV71 encephalitis (5.2%) as compared to that of the patients with EV71-related HFMD without complications (15%, OR = 0.310, 95% CI = 0.127-0.756, p = 0.006). Furthermore, homozygotes with the T allele had significantly higher levels of C-reactive protein, white blood cell count, and neutrophil count as compared to the patients with CC+CT genotypes (p = 0.004, 0.001, and 0.000, respectively). These findings suggested that the IL-17F 7488C allele could be significantly associated with protection against encephalitis in Chinese patients with EV71-related HFMD.
肠道病毒 71 型（EV71）是手足口病（HFMD）的常见病原体之一，与包括脑炎在内的严重并发症有关。白细胞介素（IL）-17F 通过诱导促炎细胞因子和趋化因子的释放，在组织炎症中发挥重要作用。我们研究了 EV71 脑炎与 IL-17F 7488T/C（rs763780）基因多态性之间的关系，该多态性已知会导致氨基酸 161 处的组氨酸到精氨酸取代。该研究在 58 例中国 EV71 脑炎患者和 127 例无并发症的中国 EV71 相关 HFMD 患者中进行。通过聚合酶链反应-限制性片段长度多态性技术确定基因型。与无并发症的 EV71 相关 HFMD 患者（27.6%，p=0.008）相比，EV71 脑炎患者的 IL-17F 7488TC+CC 基因型频率明显较低（10.3%）。EV71 脑炎患者的 IL-17F 7488C 等位基因频率也明显低于无并发症的 EV71 相关 HFMD 患者（15%，OR=0.310，95%CI=0.127-0.756，p=0.006）。此外，与 CC+CT 基因型患者相比，携带 T 等位基因的纯合子的 C 反应蛋白、白细胞计数和中性粒细胞计数明显较高（p=0.004、0.001 和 0.000）。这些发现表明，IL-17F 7488C 等位基因可能与中国 EV71 相关 HFMD 患者的脑炎保护显著相关。
