Komine Y, Inokuchi H
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
FEBS Lett. 1990 Oct 15;272(1-2):55-7. doi: 10.1016/0014-5793(90)80447-q.
The tRNA(Thr2) isoacceptor of E. coli has a G-A mismatch at positions 27-43. When the anticodon of this tRNA was converted to an amber anticodon (CUA), this tRNA showed suppressor activity in E. coli. Moreover, introduction of the base pair (G-C or U-A) at positions 27-43 of this suppressor tRNA reduced its suppressor activity. These results indicate that the G27-A43 mismatch is necessary for full function of tRNA(Thr2).
大肠杆菌的tRNA(Thr2)同功受体在27 - 43位存在一个G - A错配。当该tRNA的反密码子转变为琥珀色反密码子（CUA）时，此tRNA在大肠杆菌中表现出抑制活性。此外，在这种抑制性tRNA的27 - 43位引入碱基对（G - C或U - A）会降低其抑制活性。这些结果表明，G27 - A43错配对于tRNA(Thr2)的完整功能是必需的。
