Department of Pediatrics, Ludwig-Maximilians-University, Lindwurmstr 2a, 80337 Munich, Germany.
Pharm Res. 2012 May;29(5):1308-18. doi: 10.1007/s11095-012-0682-z. Epub 2012 Jan 21.
Targeted delivery of aerosols could not only improve efficacy of inhaled drugs but also reduce side effects resulting from their accumulation in healthy tissue. Here we investigated the impact of magnetized aerosols on model drug accumulation and transgene expression in magnetically targeted lung regions of unanesthetized mice.
Solutions containing superparamagnetic iron oxide nanoparticles (SPIONs) and model drugs (fluorescein or complexed plasmid DNA) were nebulized to unanesthetized mice under the influence of an external magnetic gradient directed to the lungs. Drug accumulation and transgene expression was subsequently measured at different time points.
We could demonstrate 2-3 fold higher accumulation of the model drug fluorescein and specific transgene expression in lung regions of mice which had been exposed to an external magnetic gradient during nebulization compared to the control mice without any exposure to magnetic gradient.
Magnetized aerosols present themselves as an efficient approach for targeted pulmonary delivery of drugs and gene therapeutic agents in order to treat localized diseases of the deeper airways.
气溶胶的靶向输送不仅可以提高吸入药物的疗效，还可以减少其在健康组织中积累所导致的副作用。在这里，我们研究了磁化气溶胶对未麻醉小鼠的磁性靶向肺部区域中模型药物积累和转基因表达的影响。
将含有超顺磁氧化铁纳米粒子（SPIONs）和模型药物（荧光素或复合质粒 DNA）的溶液在指向肺部的外部磁场梯度的影响下雾化到未麻醉的小鼠中。随后在不同时间点测量药物积累和转基因表达。
与未暴露于磁场梯度的对照小鼠相比，在雾化过程中暴露于外部磁场梯度的小鼠肺部模型药物荧光素和特异性转基因表达的积累增加了 2-3 倍。
磁化气溶胶为治疗深部气道的局部疾病提供了一种有效的靶向肺部输送药物和基因治疗剂的方法。
