Alabsi Aied M, Bakar Siti Aishah Abu, Ali Rola, Omar Abdul Rahman, Bejo Mohd Hair, Ideris Aini, Ali Abdul Manaf
Department of Biotechnology, Faculty of Agriculture and Biotechnology, University Sultan Zainal Abidin (UniSZA), Terengganu 20400, Malaysia.
Int J Mol Sci. 2011;12(12):8645-60. doi: 10.3390/ijms12128645. Epub 2011 Nov 30.
Newcastle disease virus (NDV) is used as an antineoplastic agent in clinical tumor therapy. It has prompted much interest as an anticancer agent because it can replicate up to 10,000 times better in human cancer cells than in most normal cells. This study was carried out to determine the oncolytic potential of NDV strain AF2240 and V4-UPM on WEHI-3B leukemia cell line. Results from MTT cytotoxicity assay showed that the CD(50) values for both strains were 2 and 8 HAU for AF2240 and V4-UPM, respectively. In addition, bromodeoxyuridine (BrdU) and trypan blue dye exclusion assays showed inhibition in cell proliferation after different periods. Increase in the cellular level of caspase-3 and detection of DNA laddering using agarose gel electrophoresis on treated cells with NDV confirmed that the mode of cell death was apoptosis. In addition, flow-cytometry analysis of cellular DNA content showed that the virus caused an increase in the sub-G1 region (apoptosis peaks). In conclusion, NDV strains AF2240 and V4-UPM caused cytolytic effects against WEHI-3B leukemic cell line.
新城疫病毒（NDV）在临床肿瘤治疗中用作抗肿瘤药物。作为一种抗癌药物，它引发了人们的极大兴趣，因为它在人类癌细胞中的复制能力比在大多数正常细胞中强多达10000倍。本研究旨在确定NDV毒株AF2240和V4-UPM对WEHI-3B白血病细胞系的溶瘤潜力。MTT细胞毒性试验结果表明，AF2240和V4-UPM这两种毒株的半数细胞毒性剂量（CD(50)）值分别为2血凝单位（HAU）和8 HAU。此外，溴脱氧尿苷（BrdU）和台盼蓝染料排除试验表明，在不同时间段后细胞增殖受到抑制。用NDV处理细胞后，caspase-3细胞水平升高以及使用琼脂糖凝胶电泳检测到DNA梯状条带，证实细胞死亡方式为凋亡。此外，细胞DNA含量的流式细胞术分析表明，该病毒导致亚G1区（凋亡峰）增加。总之，NDV毒株AF2240和V4-UPM对WEHI-3B白血病细胞系具有溶细胞作用。
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