Farza H, Hadchouel M, Scotto J, Tiollais P, Babinet C, Pourcel C
Unité de Recombinaison et Expression Génétique (INSERM U163, CNRS UA 271), Institut Pasteur, Paris, France.
J Virol. 1988 Nov;62(11):4144-52. doi: 10.1128/JVI.62.11.4144-4152.1988.
We have sought to address the problem of the host and tissue specificity of the hepatitis B virus (HBV) by using transgenic mice obtained after injection of head-to-tail dimers of the HBV genome. Viral DNA replication and protein synthesis were obtained in one of nine transgenic mice containing integrated HBV DNA. The RNAs encoding the HBV surface antigen and the core antigen were synthesized in the liver, the kidney, and the heart. In these organs, DNA replicative intermediates similar to those found during normal infection were associated with corelike structures. Large amounts of core polypeptides and capsids were detected in the nuclei in the absence of any pathological effect. These results show that the different steps of HBV multiplication can take place in nonliver nonhuman cells once the problem of entry into the host cell is overcome. In the absence of a small laboratory animal infectable by HBV, such transgenic mice should be helpful for the study of many aspects of viral multiplication.
我们试图通过使用注射了乙肝病毒（HBV）基因组头对头二聚体后获得的转基因小鼠来解决乙肝病毒的宿主和组织特异性问题。在九只整合了HBV DNA的转基因小鼠中，有一只实现了病毒DNA复制和蛋白质合成。编码乙肝病毒表面抗原和核心抗原的RNA在肝脏、肾脏和心脏中合成。在这些器官中，与正常感染期间发现的类似的DNA复制中间体与类核心结构相关联。在没有任何病理效应的情况下，在细胞核中检测到大量的核心多肽和衣壳。这些结果表明，一旦克服了进入宿主细胞的问题，乙肝病毒增殖的不同步骤可以在非肝脏的非人类细胞中发生。在没有可被乙肝病毒感染的小型实验动物的情况下，这种转基因小鼠应该有助于研究病毒增殖的许多方面。
