Collins M D, Williams A M, Wallbanks S
Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, U.K.
FEMS Microbiol Lett. 1990 Aug;58(3):255-62. doi: 10.1016/s0378-1097(05)80004-7.
The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogenetically distinct group. Within this pediococcal cluster, P. acidilactici was closely related to P. pentosaceus whereas P. damnosus showed a specific relationship with P. parvulus. The species P. dextrinicus, although showing significant sequence relatedness with these pediococcal species, was peripheral to the genus. Pediococcus halophilus exhibited low sequence homology with all of the species examined and formed a distinct line of descent. Pediococcus halophilus exhibited a closer affinity with enterococci and carnobacteria than with the other lactic acid bacteria. Pediococcus urinae-equi was phylogenetically very closely related to Aerococcus viridans. The 16S rRNA sequences of the type strains of these species differed by only two nucleotides (99.9% sequence homology) and clearly demonstrate that P. urinae-equi is a member of the genus Aerococcus.
利用16S rRNA的逆转录酶测序研究了片球菌属和气球菌属之间的系统发育关系。发现片球菌属在系统发育上具有异质性。嗜酸片球菌、有害片球菌、微小片球菌和戊糖片球菌这四个种形成了一个系统发育上独特的类群。在这个片球菌簇中，嗜酸片球菌与戊糖片球菌密切相关，而有害片球菌与微小片球菌呈现出特定的关系。糊精片球菌这个种，尽管与这些片球菌种表现出显著的序列相关性，但处于该属的边缘位置。嗜盐片球菌与所有检测的种表现出低序列同源性，并形成了一个独特的谱系。嗜盐片球菌与肠球菌和肉杆菌的亲缘关系比与其他乳酸菌的亲缘关系更近。尿道片球菌在系统发育上与绿色气球菌密切相关。这些种的模式菌株的16S rRNA序列仅相差两个核苷酸（序列同源性为99.9%），清楚地表明尿道片球菌是气球菌属的一个成员。
