Department of Paediatric Dentistry and Special Care, PaeCaMed research, Ghent University, Belgium.
Int J Paediatr Dent. 2013 Jan;23(1):13-22. doi: 10.1111/j.1365-263X.2011.01218.x. Epub 2012 Jan 25.
Optimal pit and fissure sealing is determined by surface preparation techniques and choice of materials.
This study aimed (i) to compare the microleakage and penetration depth of a hydrophilic sealant and a conventional resin-based sealant using one of the following preparation techniques: acid etching (AE) only, a diamond bur + AE, and Er:YAG laser combined with AE, and (ii) to evaluate the microleakage and penetration depth of the hydrophilic pit and fissure sealant on different surface conditions.
Eighty recently extracted 3rd molars were randomly assigned to eight groups of ten teeth according to the material, preparation technique, and surface condition. For saliva contamination, 0.1 mL of fresh whole human saliva was used. All samples were submitted to 1000 thermal cycles and immersed in 2% methylene blue dye for 4 h. Sections were examined by a light microscope and analysed using image analysis software (Sigmascan(®)).
The combination of Er:YAG + AE + conventional sealant showed the least microleakage. The sealing ability of the hydrophilic sealant was influenced by the surface condition.
Er:YAG ablation significantly decreased the microleakage at the tooth-sealant interface compared to the non-invasive technique. The hydrophilic sealant applied on different surface conditions showed comparable result to the conventional resin-based sealant.
窝沟封闭的效果取决于表面处理技术和材料的选择。
本研究旨在（i）比较亲水型窝沟封闭剂和传统树脂型窝沟封闭剂在以下表面处理技术下的微渗漏和渗透深度：仅酸蚀、金钢砂车针+酸蚀、Er:YAG 激光+酸蚀；（ii）评估亲水型窝沟封闭剂在不同表面条件下的微渗漏和渗透深度。
从最近拔除的 80 颗第三磨牙中随机选取 80 颗，根据材料、处理技术和表面状况分为 8 组，每组 10 颗。对于唾液污染，使用 0.1 mL 新鲜全人唾液。所有样本均经过 1000 次热循环，并用 2%亚甲蓝染料浸泡 4 小时。通过光显微镜检查切片，并使用图像分析软件（Sigmascan(®)）进行分析。
Er:YAG+AE+传统封闭剂的联合使用显示出最小的微渗漏。亲水封闭剂的密封能力受表面状况影响。
与非侵入性技术相比，Er:YAG 消融显著降低了牙-封闭剂界面的微渗漏。亲水型窝沟封闭剂在不同表面条件下的应用效果与传统树脂型窝沟封闭剂相当。
