Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU, UK.
J Am Chem Soc. 2012 Jan 25;134(3):1446-9. doi: 10.1021/ja2101196. Epub 2012 Jan 13.
A system for multistep DNA-templated synthesis is controlled by the sequential formation of DNA junctions. Reactants are attached to DNA adapters which are brought together by hybridization to DNA template strands. This process can be repeated to allow sequence-controlled oligomer synthesis while maintaining a constant reaction environment, independent of oligomer length, at each reaction step. Synthesis can take place in a single pot containing all required reactive monomers. Different oligomers can be synthesized in parallel in the same vessel, and the products of parallel synthesis can be ligated, reducing the number of reaction steps required to produce an oligomer of a given length.
多步 DNA 模板合成系统受 DNA 连接的顺序形成控制。反应物连接到 DNA 接头，通过与 DNA 模板链杂交将它们聚集在一起。该过程可以重复进行，从而允许在每个反应步骤中保持恒定的反应环境，而与寡聚物长度无关，进行序列控制的寡聚物合成。合成可以在单个容器中进行，该容器中包含所有必需的反应性单体。可以在同一容器中并行合成不同的寡聚物，并且可以将平行合成的产物进行连接，从而减少产生给定长度的寡聚物所需的反应步骤数。
