免疫球蛋白M抗甲型肝炎病毒检测：重新定位梯度离心法用于急性甲型肝炎的诊断
Immunoglobulin M anti-hepatitis A virus determination reorienting gradient centrifugation for diagnosis of Acute Hepatitis A.
作者信息
Frösner G G, Scheid R, Wolf H, Deinhardt F
出版信息
J Clin Microbiol. 1979 Apr;9(4):476-8. doi: 10.1128/jcm.9.4.476-478.1979.
Abstract
The persistence of antibody to hepatitis A antigen (anti-HAV) of the immunoglobulin M (IgM) class was evaluated in 88 sera of 51 acute hepatitis A patients. IgM was separated from IgG by a 2-h reorienting sucrose gradient ultracentrifugation, and the titer of anti-HAV was determined in the IgG- and IgM-containing fractions by solid-hase radioimmunoassay. IgM anti-HAV was the predominating antibody at onset of jaundice and persisted in these patients for at least 60 days, but not longer than 115 days. The demonstration of IgM anti-HAV is therefore a valuable tool for the diagnosis of recent hepatitis A infection.
摘要
在51例急性甲型肝炎患者的88份血清中评估了免疫球蛋白M（IgM）类抗甲型肝炎抗原抗体（抗-HAV）的持续时间。通过2小时的重定向蔗糖梯度超速离心将IgM与IgG分离，并通过固相放射免疫测定法测定含IgG和IgM的组分中的抗-HAV滴度。IgM抗-HAV是黄疸出现时的主要抗体，并在这些患者中持续至少60天，但不超过115天。因此，IgM抗-HAV的检测是诊断近期甲型肝炎感染的有价值工具。
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