Department of Chemistry, Soongsil University, Seoul 156-743, Korea.
Anal Chem. 2012 Mar 6;84(5):2172-8. doi: 10.1021/ac2024188. Epub 2012 Feb 22.
We investigated in vitro and in vivo glutathione (GSH)-induced intracellular thiopurine anticancer drug release on gold nanoparticle (Au NP) surfaces by means of label-free confocal Raman spectroscopy. Direct monitoring of GSH-triggered release of 6-mercaptopurine (6MP) and 6-thioguanine (6TG) was achieved in real time. Live cell imaging technique provides a nanomolar range release of 6MP and 6TG from Au NP surfaces after the injection of external GSH. In vivo SERS spectra of 6TG were obtained from the subcutaneous sites in living mice after GSH treatment. GSH-triggered releases of Cy5-dye assembled on 6TG-capped Au NPs were also compared using independent fluorescence measurements. Our work demonstrates that the time-lapse Raman spectroscopic tools are useful for monitoring of the controlled release of thiopurine drug molecules in vitro and in vivo.
我们通过无标记共聚焦拉曼光谱研究了金纳米粒子（Au NP）表面上谷胱甘肽（GSH）诱导的细胞内巯基嘌呤抗癌药物的体外和体内释放。实时直接监测了 6-巯基嘌呤（6MP）和 6-硫鸟嘌呤（6TG）的 GSH 触发释放。活细胞成像技术在注射外源性 GSH 后，从 Au NP 表面提供了纳摩尔级的 6MP 和 6TG 释放。用 GSH 处理后，从活小鼠的皮下部位获得了 6TG 的体内 SERS 光谱。还使用独立的荧光测量比较了组装在 6TG 封端的 Au NPs 上的 Cy5 染料的 GSH 触发释放。我们的工作表明，时移拉曼光谱工具可用于体外和体内监测巯基嘌呤药物分子的控制释放。
