Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden.
FEMS Microbiol Ecol. 2012 Jun;80(3):556-65. doi: 10.1111/j.1574-6941.2012.01317.x. Epub 2012 Feb 17.
In vector mosquitoes, the presence of midgut bacteria may affect the ability to transmit pathogens. We have used a laboratory colony of Aedes aegypti as a model for bacterial interspecies competition and show that after a blood meal, the number of species (culturable on Luria-Bertani agar) that coexist in the midgut is low and that about 40% of the females do not harbor any cultivable bacteria. We isolated species belonging to the genera Bacillus, Elizabethkingia, Enterococcus, Klebsiella, Pantoea, Serratia, and Sphingomonas, and we also determined their growth rates, antibiotic resistance, and ex vivo inhibition of each other. To investigate the possible existence of coadaptation between midgut bacteria and their host, we fed Ae. aegypti cohorts with gut bacteria from human, a frog, and two mosquito species and followed the bacterial population growth over time. The dynamics of the different species suggests coadaptation between host and bacteria, and interestingly, we found that Pantoea stewartii isolated from Ae. aegypti survive better in Ae. aegypti as compared to P. stewartii isolated from the malaria mosquito Anopheles gambiae.
在媒介蚊子中，肠道细菌的存在可能会影响传播病原体的能力。我们使用埃及伊蚊的实验室品系作为细菌种间竞争的模型，结果表明，在吸血后，肠道中共存的物种数量（可在 LB 琼脂上培养）较少，约有 40%的雌性蚊子不携带任何可培养细菌。我们分离到属于芽孢杆菌属、伊丽莎白菌属、肠球菌属、克雷伯氏菌属、泛菌属、沙雷氏菌属和鞘氨醇单胞菌属的物种，并测定了它们的生长速度、抗生素耐药性以及彼此的体外抑制作用。为了研究肠道细菌与其宿主之间可能存在的共同适应，我们用人类、青蛙和两种蚊子的肠道细菌喂食埃及伊蚊种群，并随时间跟踪细菌种群的生长情况。不同物种的动态表明了宿主和细菌之间的共同适应，有趣的是，我们发现从埃及伊蚊中分离出的潘氏不动杆菌与从疟蚊冈比亚按蚊中分离出的潘氏不动杆菌相比，在埃及伊蚊中存活得更好。
