Shah Masaud, Shams Sulaiman, Rahman Ziaur
Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Genet Vaccines Ther. 2012 Jan 30;10(1):1. doi: 10.1186/1479-0556-10-1.
Countrywide 5.9 million, 0-11 Month old children are immunized annually by EPI (Expended Program on Immunization) against 8 vaccine preventable diseases including measles and so on. Unfortunately the basic immunity centers are not uniform throughout the country. Each center provides services to about 27000 people which is inadequate. The purpose of this study was to explore the development of EPI Pakistan in terms of immunization of measles.
Nucleotide sequences were analyzed by neighbor joining method (bootstrap test) using Bio- edit and MEGA-5 software to find evolutionary relationship between wild type measles strain and vaccine strain (Edmonston strain) used in Pakistan. For statistical analysis of data SPSS 16 was used.
Currently 1.3 vaccinators are working at each U C (union council) which according to national EPI policy should be at least 2. About 56% and 44% children of age 0-11 months did not received second dose of measles in the last two years respectively. Out of these 4231 cases which were reported last year, 1370 have received their first dose of measles vaccine.
Seroconversion and seroprevalence study of the vaccine and field strain of measles virus is needed to confirm whether its failure is due to service unavailability or vaccine in-affectivity.
全国每年有590万0至11个月大的儿童通过扩大免疫规划（EPI）接种疫苗，预防包括麻疹等8种疫苗可预防疾病。不幸的是，全国基本免疫中心并不统一。每个中心为约27000人提供服务，这是不够的。本研究的目的是从麻疹免疫方面探讨巴基斯坦扩大免疫规划的发展情况。
使用Bio-edit和MEGA-5软件通过邻接法（自展检验）分析核苷酸序列，以找出巴基斯坦使用的野生型麻疹毒株与疫苗株（埃德蒙斯顿株）之间的进化关系。数据统计分析使用SPSS 16。
目前每个联合委员会（UC）有1.3名接种员，而根据国家扩大免疫规划政策，至少应有2名。在过去两年中，分别约有56%和44%的0至11个月大儿童未接种第二剂麻疹疫苗。在去年报告的这4231例病例中，有1370例接种了第一剂麻疹疫苗。
需要对麻疹病毒疫苗株和现场毒株进行血清转化和血清流行率研究，以确认其失败是由于服务不可用还是疫苗无效。
