评估肥胖和非肥胖女性的血浆 MMP-8、MMP-9、TIMP-1 和 MPO 水平。
Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women.
机构信息
Núcleo de Pós-Graduação e Pesquisa, Santa Casa de Belo Horizonte, Rua Domingos Vieira 590, Belo Horizonte, MG, Brazil.
出版信息
Clin Biochem. 2012 Apr;45(6):412-5. doi: 10.1016/j.clinbiochem.2012.01.008. Epub 2012 Jan 18.
OBJECTIVES
To compare the plasma concentrations of matrix metalloproteinase (MMP)-9, tissue inhibitor of MMP (TIMP)-1, MMP-8, and myeloperoxidase (MPO) for obese and lean women.
DESIGN AND METHODS
We recruited 30 lean and 36 obese women without comorbidities. The MMP-9, TIMP-1, and MMP-8 levels were measured using enzyme-linked immunosorbent assay (ELISA). MPO activity was assessed by a colorimetric assay.
RESULTS
Obese women had higher MMP-9 levels and MMP-9:TIMP-1 ratios than lean women. Conversely, the MMP-8 levels and MMP-8:TIMP-1 ratios in the obese women were significantly lower than those in the lean women despite neutrophil activation, which was assessed by MPO activity.
CONCLUSION
We observed that MMP-9 and MMP-8 had distinct profiles, which suggested that these 2 enzymes play different roles in obesity.
目的
比较肥胖和非肥胖女性的基质金属蛋白酶（MMP）-9、基质金属蛋白酶抑制剂（TIMP）-1、MMP-8 和髓过氧化物酶（MPO）的血浆浓度。
设计和方法
我们招募了 30 名非肥胖女性和 36 名肥胖女性，她们没有合并症。采用酶联免疫吸附试验（ELISA）检测 MMP-9、TIMP-1 和 MMP-8 水平。通过比色法评估 MPO 活性。
结果
肥胖女性的 MMP-9 水平和 MMP-9/TIMP-1 比值高于非肥胖女性。然而，尽管 MPO 活性评估提示中性粒细胞激活，肥胖女性的 MMP-8 水平和 MMP-8/TIMP-1 比值明显低于非肥胖女性。
结论
我们观察到 MMP-9 和 MMP-8 具有不同的特征，表明这两种酶在肥胖中发挥不同的作用。
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