Institute of Sports Medicine, Department of Orthopedic Surgery M81, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark; Department of Endocrinology and Internal Medicine, Aarhus University Hospital and The Medical Research Laboratories, Institute of Clinical Medicine, Faculty of Health Sciences, Aarhus University, Aarhus, Denmark.
Scand J Med Sci Sports. 2013 Oct;23(5):614-9. doi: 10.1111/j.1600-0838.2011.01431.x. Epub 2012 Jan 31.
Collagen is the predominant structural protein in tendons and ligaments, and can be controlled by hormonal changes. In animals, injections of insulin-like growth factor I (IGF-I) has been shown to increase collagen synthesis in tendons and ligaments and to improve structural tissue healing, but the effect of local IGF-I administration on tendon collagen synthesis in human has not been studied. The purpose of this study was to study whether local injections of IGF-I would have a stimulating effect on tendon collagen synthesis. Twelve healthy nonsmoking men [age 62 ± 1 years (mean ± SEM), BMI 27 ± 1] participated. Two injections of either human recombinant IGF-I (0.1 mL Increlex©) or saline (control) into each patellar tendon were performed 24-h apart, respectively. Tendon collagen fractional synthesis rate (FSR) was measured by stable isotope technique in the hours after the second injection. Simultaneously, interstitial peritendinous (IGF-I) and [procollagen type I N-terminal propeptide (PINP)], as a marker for type I collagen synthesis, were determined by microdialysis technique. Tendon collagen FSR and PINP were significantly higher in the IGF-I leg compared with the control leg (P < 0.05). In conclusion, local IGF-I administration can directly enhance tendon collagen synthesis both within and around the human tendon tissue.
胶原蛋白是肌腱和韧带中的主要结构蛋白，可受激素变化的控制。在动物中，注射胰岛素样生长因子 I（IGF-I）已被证明可增加肌腱和韧带中的胶原蛋白合成，并改善结构组织愈合，但局部 IGF-I 给药对人类肌腱胶原蛋白合成的影响尚未研究。本研究旨在研究局部注射 IGF-I 是否会对肌腱胶原蛋白合成产生刺激作用。12 名健康不吸烟男性[年龄 62 ± 1 岁（平均值 ± SEM），BMI 27 ± 1]参与了研究。分别在 24 小时内对每个髌腱进行两次注射，分别为 IGF-I（0.1 毫升 Increlex©）或生理盐水（对照）。在第二次注射后的几个小时内，通过稳定同位素技术测量肌腱胶原蛋白的分数合成率（FSR）。同时，通过微透析技术测定间质周围（IGF-I）和[I 型前胶原 N 端前肽（PINP）]，作为 I 型胶原蛋白合成的标志物。IGF-I 组的肌腱胶原蛋白 FSR 和 PINP 明显高于对照组（P <0.05）。总之，局部 IGF-I 给药可直接增强人类肌腱组织内和周围的肌腱胶原蛋白合成。
