Centre National de la Résistance aux Antibiotiques, Laboratoire associé Pseudomonas aeruginosa, Hôpital Jean Minjoz, Besançon, France.
Antimicrob Agents Chemother. 2012 Apr;56(4):2187-90. doi: 10.1128/AAC.05838-11. Epub 2012 Jan 30.
Analysis of two clonally related multiresistant Pseudomonas aeruginosa isolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). The bla(IMP-29) gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems.
对两株克隆相关的多耐药铜绿假单胞菌分离株的分析导致了一种新型 IMP 型金属β-内酰胺酶的鉴定。IMP-29 与其他 IMP 变体（最接近的变体是 IMP-5，氨基酸同一性为 93%）有显著差异。bla(IMP-29)基因盒位于 10.298 株的 1 类整合子中，而在 10.266 株中，它位于一个 30kb 可接合质粒的重排 DNA 区域。生化分析证实，IMP-29 能有效水解碳青霉烯类抗生素。
