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利用聚合酶链反应对人类免疫缺陷病毒DNA进行定量分析。

Quantitation of human immunodeficiency virus DNA by using the polymerase chain reaction.

作者信息

Dickover R E, Donovan R M, Goldstein E, Dandekar S, Bush C E, Carlson J R

机构信息

Department of Internal Medicine, University of California, Davis 95616.

出版信息

J Clin Microbiol. 1990 Sep;28(9):2130-3. doi: 10.1128/jcm.28.9.2130-2133.1990.

DOI:10.1128/jcm.28.9.2130-2133.1990
PMID:2229398
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268121/
Abstract

The polymerase chain reaction was used to measure the DNA copy number of human immunodeficiency virus (HIV). Differences in polymerase chain reaction amplification efficiency were controlled by amplifying known amounts of HIV DNA in parallel with samples. This technique is a sensitive, accurate, and reproducible method for the quantitation of HIV DNA.

摘要

聚合酶链反应被用于测量人类免疫缺陷病毒（HIV）的DNA拷贝数。通过与样本平行扩增已知量的HIV DNA来控制聚合酶链反应扩增效率的差异。该技术是一种用于定量HIV DNA的灵敏、准确且可重复的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b89b/268121/ea39ca3cd6d5/jcm00057-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b89b/268121/ea39ca3cd6d5/jcm00057-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b89b/268121/ea39ca3cd6d5/jcm00057-0270-a.jpg

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