Hoffman S A, Madsen C S
Department of Microbiology, Arizona State University, Tempe 85287-2701.
J Neuroimmunol. 1990 Dec;30(2-3):229-37. doi: 10.1016/0165-5728(90)90107-x.
Autoantibodies which bind to integral membrane proteins of brain were tested for their ability to bind to cross-reactive antigens on non-neural tissue. Both brain specific autoantibodies and antibodies which bind to cross-reactive antigens were found. There were two types of brain reactive autoantibodies which could not be adsorbed by non-neural tissue. One type was adsorbable by crude cell membrane preparations of brain. The second type was reactive against integral membrane proteins of brain, but not adsorbable by any of the crude membrane preparations tested. Autoantibodies of the first type reacted against integral membrane proteins with apparent molecular weights of 75, 70, 62, 50, 27, 24 and 20 kDa, as determined by gel electrophoresis and immunoblotting. As in previous studies, a diversity of brain reactive autoantibodies were found. The greatest numbers and strongest banding patterns were seen in the autoimmune strains of mice. The non-autoimmune strain displayed these autoantibodies at much lower levels. These results are the first to find brain specific autoantibodies, from autoimmune mice, against integral brain membrane antigens. The data support the idea that there is a sub-population of brain reactive autoantibodies which are involved in the pathogenesis of neuropsychiatric manifestations in immunologic disorders, particularly systemic lupus erythematosus.
检测了与脑的整合膜蛋白结合的自身抗体与非神经组织上交叉反应抗原的结合能力。发现了脑特异性自身抗体以及与交叉反应抗原结合的抗体。有两种类型的脑反应性自身抗体不能被非神经组织吸附。一种类型可被脑的粗制细胞膜制剂吸附。第二种类型与脑的整合膜蛋白反应，但不能被所测试的任何粗制膜制剂吸附。通过凝胶电泳和免疫印迹法测定，第一种类型的自身抗体与表观分子量为75、70、62、50、27、24和20 kDa的整合膜蛋白反应。如先前研究一样，发现了多种脑反应性自身抗体。在自身免疫性小鼠品系中观察到数量最多且条带模式最强。非自身免疫性品系中这些自身抗体的水平要低得多。这些结果首次从自身免疫性小鼠中发现了针对脑整合膜抗原的脑特异性自身抗体。数据支持这样一种观点，即存在一部分脑反应性自身抗体参与免疫性疾病，特别是系统性红斑狼疮的神经精神表现的发病机制。
