Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, Korea.
Planta Med. 2012 Mar;78(5):485-7. doi: 10.1055/s-0031-1298215. Epub 2012 Jan 31.
Three new diterpenoids, 18-formyloxy-8 β-hydroxysandaracopimar-15-ene (1), 15(R)-N-butoxypinusolidic acid (2), and 15,16-dihydro-15,16-dimethoxylambertianic acid (3), along with twelve known compounds (4-15) were isolated from MeOH extracts of leaves of Thuja orientalis L. The structures of the three new compounds were elucidated on the basis of spectroscopic analyses, including extensive 2D-NMR data. The absolute stereochemistry of compound 2 was clarified by a CD spectroscopic study. The isolated compounds were evaluated for cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines in vitro using the sulforhodamin B (SRB) bioassay.
从东方杉（Thuja orientalis L.）叶的甲醇提取物中分离得到三个新的二萜类化合物，18-甲氧基-8β-羟基松香-15-烯（1）、15(R)-N-丁氧基松柏酸（2）和 15,16-二氢-15,16-二甲氧基 Lambertianic 酸（3），以及十二个已知化合物（4-15）。基于光谱分析，包括广泛的 2D-NMR 数据，确定了这三个新化合物的结构。通过圆二色光谱研究确定了化合物 2 的绝对立体化学。采用磺基罗丹明 B（SRB）生物测定法，体外评估了分离得到的化合物对 A549、SK-OV-3、SK-MEL-2 和 HCT-15 细胞系的细胞毒性。
